METHOD OF OBTAINING BACTERIAL PRODUCER OF RECOMBINANT NC NUCLEOCAPPSID PROTEIN OF SARS-CoV-2 VIRUS Russian patent published in 2024 - IPC C12N15/00 C12N1/00 

FIELD: biotechnology.

SUBSTANCE: method of obtaining a producer of the recombinant nucleocapsid protein NC of SARS-CoV-2 virus based on the vaccine strain F. tularensis 15 “НИИЭГ” is constructing a stable recombinant plasmid pPNC-2 with a recombinant operon consisting of the promoter region of the groE gene and a fragment of the fopA gene encoding the leader sequence FopL of FopA protein of F. tularensis, and the structural part of the recombinant nc gene of SARS-CoV-2 virus. The plasmid pPNC-2 obtained in this way with the cat gene removed was transferred into the cells of the F. tularensis strain 15 “НИИЭГ” by co-transformation with the plasmid pTV24 (Tc^RCm^R) and after a three-stage selection and PCR analysis, a strain synthesizing the immunogenic recombinant nucleocapsid protein NC F. tularensis 15-NC-2 strain was selected without the additional antibiotic resistance marker cat gene. The proposed invention makes it possible to obtain a modified strain of F. tularensis that synthesizes the immunologically active recombinant nucleocapsid protein NC of SARS-CoV-2 virus.

EFFECT: strain can be used to create a prototype of a live combined recombinant vaccine for the prevention of both coronavirus infection and other infections caused by intracellular pathogens.

1 cl, 6 dwg