METHOD FOR THE DETERMINATION OF FRANCISELLA TULARENSIS SUBSPECIES BY MULTI-PRIMER PCR Russian patent published in 2022 - IPC C12N15/00 G01N33/53 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. A method for the determination of Francisella tularensis subspecies by the method of multi-primer PCR is described, including the isolation of DNA from the investigated strain of F. tularensis, PCR with specific primers and registration of the reaction using electrophoresis. The proposed method differs in that two common INDEL genes with deletions of a certain size, namely ft1779 and ft426, are detected on F. tularensis DNA, followed by their amplification using designed specific primers: to the ft1779 gene - primers GCCTTTTCAGTTCTTGAAATTGT and TTTGAGATTCGTGTAGTGTACTTGTG, with the amplified fragment length of 98 bps, or 107 bps, or 950 bps, to the ft426 gene - the primers GATTACACGACTACG, with the amplified fragment length of 98 bps, or 107 bps, or 950 bps, to the ft426 gene - the primers GATTACCACGACCTAG and the amplified fragment of 174 bps or 196 bps.

EFFECT: invention expands the arsenal of methods for determining the subspecies of Francisella tularensis by multi-primer PCR.

3 cl, 1 dwg, 1 tbl, 4 ex