FIELD: chemistry.
SUBSTANCE: displayed polypeptides include at least one unnatural aryl-azide amino acid such as, for example, para-azido-L-phenylalanine, which is incorporated into the phage-displayed hybrid polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA.
EFFECT: invention enables to modify protein in physiological conditions in which phage activity and survival is maintained.
19 cl, 16 dwg, 9 ex
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