GENETIC CODED FLUORESCENT COUMARINIC AMINO ACIDS Russian patent published in 2013 - IPC C12N15/54 C12N9/10 C12N15/74 C12P21/00 

FIELD: medicine.

SUBSTANCE: what is presented is an orthogonal pair comprising tRNA (O-tRNA) and aminoacyl-tRNA synthetase (O-RS) wherein O-tRNA is suppressive tRNA recognizing an amber codon, while O-RS represents a mutant form of tyrosyl-tRNA synthetase Methanococcus janaschii, preferentially aminoacylating said tRNA by unnatural amino acid L-(7-hydroxycoumarin-4-yl)-ethyl glycine. What is described is a translation system providing the introduction in any specified position of a protein of interest synthetised in this system, of unnatural amino acid L-(7-hydroxycoumarin-4-yl)-ethyl glycine which comprises the orthogonal pair under the invention, said unnatural amino acid and nucleic acid which codes the protein of interest and comprises a selector codon recognised by O-tRNA in the specified position.

EFFECT: method improvement.

20 cl, 9 dwg, 5 ex