METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS OF HEAVY AND LIGHT CHAINS OF HUMAN IMMUNOGLOBULINS FOR THE CREATION OF RECOMBINANT ANTIBODIES, PRIMER COMPOSITIONS (VARIANTS) Russian patent published in 2022 - IPC C12N5/78 C12Q1/68 C12Q1/6806 

Abstract RU 2775914 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology, in particular to the method for amplification of nucleic acids of the heavy and light chain of human immunoglobulins.

EFFECT: invention makes it possible to reduce the probability of false-negative amplification, increasing the probability of successful amplification of cDNA antibodies with rarely occurring alleles of V gene segments.

1 cl, 16 dwg, 6 ex

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Authors

Marin Maksim Aleksandrovich

Firstova Viktoriya Valerevna

Shkuratova Mariya Andreevna

Kartseva Alena Sergeevna

Silkina Marina Vladimirovna

Zeninskaya Natalya Alekseevna

Ryabko Alena Konstantinovna

Romanenko Yana Olegovna

Shemyakin Igor Georgievich

Dates

2022-07-11Published

2020-11-13Filed