FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and immunology. Mouse for production of immunoglobulin chain is disclosed. Mouse is capable to produce immunoglobulin chain contains in its genome variable light chain domain, fused with constant domain of heavy chain, containing in its germ cells first non-rearranged Vκ-segment of human light chain and non-rearranged human Jκ-segment, functionally connected to endogenous nucleotide sequence of constant mouse heavy chain in endogenous mouse locus of heavy chain. Wherein first non-rearranged Vκ gene segment of human light chain and non-rearranged human Jκ gene segment replace all functional endogenous VH gene segments of mouse heavy chain, all functional endogenous DH gene mouse segments and all functional endogenous JH gene segments of mouse heavy chain in endogenous mouse locus of heavy chain. Use of such mouse for antibody production is also described.
EFFECT: production of immunoglobulin chain.
22 cl, 21 dwg, 5 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MICE THAT PRODUCE BINDING PROTEINS CONTAINING V DOMAINS | 2011 |
|
RU2750219C2 |
| MICE EXPRESSING AN IMMUNOGLOBULIN HYBRID LIGHT CHAIN | 2011 |
|
RU2601297C2 |
| MICE, EXPRESSING LIGHT CHAIN WITH VARIABLE REGION OF ALPHA HUMAN AND CONSTANT AREA OF MOUSE | 2011 |
|
RU2590594C2 |
| HUMANIZED GNAWING ANIMALS THAT EXPRESS HEAVY CHAINS CONTAINING V DOMAINS | 2013 |
|
RU2664185C2 |
| HUMANIZED RODENTS WHICH EXPRESS HEAVY CHAINS CONTAINING VDOMAINS | 2013 |
|
RU2770016C2 |
| HUMANISED LIGHT CHAIN MICE | 2012 |
|
RU2664181C2 |
| MICE WITH HUMANIZED LIGHT CHAIN | 2012 |
|
RU2761639C2 |
| ADAM6 MICE | 2012 |
|
RU2582261C2 |
| RESTRICTED IMMUNOGLOBULIN HEAVY CHAIN MICE | 2012 |
|
RU2603090C2 |
| NON-HUMAN ANIMALS WITH CONSTRUCTED LIGHT CHAIN OF LAMBDA IMMUNOGLOBULIN, AND THEIR USE | 2018 |
|
RU2778410C2 |
