PROBE AND KIT (VERSION) FOR DETECTING TARGET NUCLEIC ACID, SPACER APPLICABLE TO BE ATTACHED TO TARGET-SPECIFIC PROBE SEQUENCE AND METHOD FOR DETECTING ANY INTERACTION OF PROBE AND TARGET NUCLEIC ACID Russian patent published in 2012 - IPC C12N15/11 C12Q1/68 

FIELD: medicine.

SUBSTANCE: invention refers to a probe for detecting and a kit (versions) for detecting target nucleic acid, a spacer applicable to be attached to a target-specific probe sequence and a method for detecting any interaction of said probe and target nucleic acid. The probe comprises: a binding group which enables binding the probe and a particle-like substrate surface; a spacer; and a target-specific oligonucleotide probe sequence. The spacer comprises: the oligonucleotide spacer of at least 20 nucleotides between the target-specific oligonucleotide probe sequence and the substance-bound group, containing the homopolymer polythymine spacer and the heteropolymer spacer of repeated nucleotide links specified in a group consisting of TTG, TTTG, AAG, AAC, AAAG and AAAC; and optionally the carbon spacer of 12 - 30 carbon links between the target-specific oligonucleotide probe sequence and the substance-bound group.

EFFECT: invention enables detecting target nucleic acid and increasing the signal-noise relation in the kits.

25 cl, 4 dwg, 42 tbl, 12 ex