METHOD FOR TESTING AND QUALITY CONTROL OF NUCLEIC ACIDS ON SUPPORT Russian patent published in 2016 - IPC C12Q1/68 

Abstract RU 2582250 C2

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Invention further relates to a kit for testing nucleic acids on a support having an array of nucleic acids immobilised on a solid support, wherein each of immobilised nucleic acids includes a portion of nucleotides of one base-type, and a labelled oligonucleotide which is complementary to said portion of nucleotides. Invention also relates to use of a labelled oligonucleotide which is complementary to portion of nucleotides of one base-type for testing condition of nucleic acids immobilised on a solid support.

EFFECT: described is a method for testing nucleic acids on a support, comprising immobilising one or more nucleic acids by crosslinking, wherein each of immobilised nucleic acids includes a portion of nucleotides of one base-type, obtaining the labelled oligonucleotides which are complementary to portion of nucleotides, and determining a parameter reflecting condition of said nucleic acids.

33 cl, 6 dwg, 5 ex

Similar patents RU2582250C2

Title Year Author Number
MODIFIED OLIGONUCLEOTIDE IMMOBILISATION ON A POLYMER SUBSTRATE BY PHYSICAL ADSORPTION 2015
  • Feitsma, Harma Martine
  • Lamberts, Inge
  • Kuijpers, Steven Paulus Lambertus
RU2732791C2
METHOD AND PRODUCT FOR LOCALISED OR SPATIAL DETECTION OF NUCLEIC ACID IN TISSUE SAMPLE 2012
  • Frisen Jonas
  • Stokhl Patrik
  • Lundeberg Joakim
RU2603074C2
DETECTION OF NUCLEIC ACID TARGET SEQUENCE IN ANALYSIS WITHOUT HYBRIDIZATION, DEPENDING ON CLEAVAGE AND ELONGATION OF THE PROBING AND LABELING OLIGONUCLEOTIDE (PTO) 2013
  • Chun Dzhon Joon
  • Li Yang Dzho
RU2620958C2
DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION ASSAY 2012
  • Chun Dzhon Joon
  • Li Jang Dzho
RU2566562C2
DETERMINATION OF TARGET NUCLEIC ACID SEQUENCES BY SPLITTING OLIGONUCLEOTIDE PROBE AND HYBRIDISATION 2012
  • Chun Dzhon Joon
  • Li Yang Dzho
RU2588457C2
SETS OF OLIGONUCLEOTIDE PROBES AND METHODS OF GASTROINTESTINAL MICROBIOTA PROFILING 2011
  • Vebe Khejdi
  • Rudi Knut
  • Sekelja Monika
RU2605913C2
MOLECULAR PROBE 1991
  • Majkl S.Urdea[Us]
RU2107730C1
TARGET-DISCRIMINATING PROBE, METHOD OF CONSTRUCTING THEREOF AND METHOD OF DETECTING NUCLEIC ACID TARGET-SEQUENCE (VERSIONS) 2010
  • Chun, Dzhon Joon
  • Khvan, In Taehk
  • Leeh, San Kil
RU2542478C2
COMPOSITIONS AND DETECTION METHODS 2008
  • Park Dehniel Dzh.
  • Kkhan Zakheer
  • Petter Karl F.
RU2480732C2
DETECTION OF NUCLEOTIDE VARIATIONS IN NUCLEIC ACID SEQUENCE-TARGETS IN THE ANALYSIS WITH THE CLEAVAGE AND EXTENSION OF THE PROBING AND TAGGING OLIGONUCLEOTIDE (PTO) 2013
  • Chun Dzhon Joon
  • Li Yang Dzho
RU2620955C2

RU 2 582 250 C2

Authors

Pirik Anke

Stapert Khendrik R.

Dates

2016-04-20Published

2009-09-29Filed