FIELD: chemistry.
SUBSTANCE: method involves culturing mammal cells containing a gene which codes the blood-coagulation factor IX, in a cell culturing medium containing approximately from 10 nM to 600 nM manganese under conditions and during a time sufficient for expression of the blood-coagulation factor IX.
EFFECT: invention enables to obtain an expressed blood-coagulation factor IX with high degree of glycosylation compared to the degree of glycosylation observed using the same conditions and the same medium but without manganese.
25 cl, 8 dwg, 4 tbl, 5 ex
