FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry and is a method comprising the steps of: (a) providing cells in a cell culture medium to initiate the cell culture process, wherein said cell culture medium contains iron as a trace element; and wherein the iron concentration is less than 100 mcM; and (b) adding a composition comprising iron to the aforementioned cell culture medium during cell culture, so that the concentration of iron in the cell culture medium is increased during the cell culture process, wherein the iron-containing composition is added on or after 3 days of the cell culture process.
EFFECT: increased density, vitality and/or titer of cells in a cell culture medium.
28 cl, 14 dwg, 3 ex
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