METHOD FOR SIMULATING INFECTED PANCREATONECROSIS Russian patent published in 2012 - IPC G09B23/28 

FIELD: medicine.

SUBSTANCE: simulating infected pancreatonecrosis is ensured by inspection of an abdominal cavity with pancreas mobilisation. The pancreas is injured in a distal portion of a right lobe transversally through 3 cm with the use of Bilroth's hemostatic forceps for three times in various places. autobile 0.2 ml and the Acinetobacter baumannii clinical strain in the amount of 10⁵ CFU/ml are introduced in a pancreatic parenchyma. Three days later, the animal is removed from the experiment; it is followed by blood and pancreatic tissue sampling and freezing at temperature 77°K. Electronic paramagnetic resonance is used to determine blood transferrin, ceruloplasmin and methemoglobin, pancreatic free iron, transferrin. If the values of methemoglobin and ceruloplasmin appear to increase to 0.16±0.05 standard units and 3.68±1.02 standard units respectively, the concentration of blood transferrin decreases to 0.83±0.24 standard units, while the level of free iron increases by 15 3 % and transferrin - by 206% as compared with the normal values, developing pancreatonecrosis is stated.

EFFECT: method enables simulating infected pancreatonecrosis by introducing autobile and pathogenetically proved and dosed agent into the pancreatic parenchyma after its injury.

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