FIELD: biotechnology.
SUBSTANCE: invention represents a non-spore-forming Bacillus cell for producing a sialylated oligosaccharide, wherein said Bacillus cell is genetically engineered to contain lactose permease, a CMP-NeuNAc biosynthesis pathway and sialyltransferase, wherein the Bacillus cell sporulation capacity is impaired as a result of deletion or functional inactivation of one or more genes coding Spo0A, sigma E and sigma F. Invention also relates to a method of producing a sialylated oligosaccharide using said Bacillus cell.
EFFECT: disclosed is a non-spore-forming Bacillus cell for producing a sialylated oligosaccharide.
13 cl, 1 dwg, 3 tbl, 6 ex
| Title |
Year |
Author |
Number |
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2020 |
- Jennevajn, Shtefan
- Vartenberg, Dirk
|
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| SIALYLTRANSFERASES AND USE THEREOF IN PRODUCING SIALYLATED OLIGOSACCHARIDES |
2018 |
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- Vartenberg, Dirk
|
RU2822039C2 |
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|
RU2810729C2 |
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2018 |
- Jennevajn, Shtefan
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|
RU2818835C2 |
| ENZYMATIC PRODUCTION OF OLIGOSACCHARIDES THROUGH GENERAL FERMENTATION USING MIXED RAW MATERIALS |
2019 |
- Jennevajn, Shtefan
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|
RU2801231C2 |
| ENZYMATIC PRODUCTION OF SIALYLATED SACCHARIDES |
2019 |
- Jennevajn, Shtefan
- Vartenberg, Dirk
|
RU2819876C2 |
| SIMPLE METHOD OF SIALILLACTOSE PURIFICATION |
2019 |
- Jennevajn, Shtefan
- Khelfrikh, Markus
- Engels, Benedikt
|
RU2793469C2 |
| USE OF GLYCOSIDASES IN OLIGOSACHARIDE PRODUCTION |
2019 |
- Jennevajn, Shtefan
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|
RU2810730C2 |
| METHOD OF PRODUCING SIALYLATED OLIGOSACCHARIDES |
2007 |
|
RU2473695C2 |
| METHOD OF PURIFYING SIALYLATED OLIGOSACCHARIDES |
2018 |
- Jennevajn, Shtefan
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- Engels, Benedikt
|
RU2799091C2 |
