FIELD: chemistry.
SUBSTANCE: invention relates to biotechnology and particularly to a bacterial cell stably cultured in a medium, where the cell is adapted to produce oligosaccharides. The cell is transformed such that it contains at least one nucleic acid sequence which encodes an enzyme involved in oligosaccharide synthesis. The cell is further transformed such that it contains at least one nucleic acid sequence which encodes a protein of the sugar efflux transporter family, or functional homologue thereof. The invention also relates to a method of producing oligosaccharides which involve said cell.
EFFECT: invention enables to obtain oligosaccharides with high efficiency.
13 cl, 3 dwg, 2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHESIS OF FUCOSYLATED COMPOUNDS | 2009 |
|
RU2584599C2 |
| FUCOSYLTRANSFERASES AND THEIR USE FOR OBTAINING FUCOSYLATED OLIGOSACCHARIDES | 2018 |
|
RU2818835C2 |
| NEW FUCOSYLTRANSFERASES AND APPLICATIONS THEREOF | 2011 |
|
RU2628307C2 |
| NEW FUCOSYLTRANSFERASES AND THEIR APPLICATION | 2011 |
|
RU2642307C2 |
| IMPROVED METHOD FOR PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES | 2017 |
|
RU2790445C2 |
| SPRAY DRIED 3-FUCOSYLLACTOSE | 2018 |
|
RU2810298C2 |
| SPRAY-DRIED BREAST MILK OLIGOSACCHARIDE MIXTURE | 2018 |
|
RU2803849C2 |
| PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES IN BACILLUS | 2020 |
|
RU2810729C2 |
| PRODUCTION OF HUMAN DAIRY OLIGOSACCHARIDES IN MICROBIAL PRODUCERS WITH ARTIFICIAL IMPORT/EXPORT | 2016 |
|
RU2798786C2 |
| PRODUCTION OF HUMAN MILK OLIGOSACCHARIDES IN HOST MICROORGANISMS WITH MODIFIED IMPORT/EXPORT | 2016 |
|
RU2763170C2 |
