VERSIONS OF LIPOLYTIC ENZYME Russian patent published in 2013 - IPC C12N9/20 C12P7/40 

FIELD: chemistry.

SUBSTANCE: invention describes a lipolytic polypeptide and a method for production thereof, involving: 1) collecting parent lipase with an amino acid sequence which is at least 80% identical to the sequence SEQ ID NO:1, which is given the description; 2) collecting at least one amino acid residue in the sequence; 3) altering the amino acid sequence, where the alteration includes one or more of the following: N74Q, P143S, A281S, P38S, N292Q, L1QGPL, L1QL, I285E, L147F, L147N, N292C, L140E, P143L, A146T, P280V, A283K, A284N, T103G + A148P, W104H + A148P, N74Q + A281S, V190A, L199P, T256K, T42N, R242A, V2151, T164V, L163F + T164V, D265P, P303K, R168D, A25G, V315I, T244P, K13Q, L277I, Y91S + A92S + N96S + N97* + L99V or D223G; 4) obtaining the altered polypeptide with the altered amino acid sequence; 5) determining specific activity of the lipolytic enzyme; and 6) collecting the altered polypeptide, having higher specific activity of the lipolytic enzyme than the parent polypeptide. The invention also discloses methods of conducting reactions which are catalysed by the described lipase, which involve reaction of reactants with said polypeptide, where the reaction is: hydrolysis of a carboxylic ester, synthesis of a carboxylic ester, alcoholysis of a carboxylic ester or acidolysis of a carboxylic ester.

EFFECT: invention enables to obtain lipase with higher specific activity than the parent lipase.

9 cl, 5 tbl, 1 dwg, 8 ex