FIELD: chemistry.
SUBSTANCE: invention relates to enzymatic techniques of removing phospholipids from plant oil. The method of degumming an oil composition involves contacting the oil composition containing phospholipids simultaneously with one or more phospholipase A (PLA) enzymes in amount of about 2 ppm of the active enzyme or less and one or more phospholipase C (PLC) enzymes in amount of about 30 ppm of the active enzyme or less. Reaction of the enzymes with phospholipids takes place at pH of about 3-7, at temperature of about 40-80°C and the reaction takes less than one hour. The reaction products are separated from the oil composition. A method of degumming oil is also disclosed.
EFFECT: obtained degummed oil composition has phospholipid content, measured in parts per million of phosphorus, of about 20 ppm or less.
23 cl, 7 dwg, 7 tbl, 38 ex
| Title | Year | Author | Number |
|---|---|---|---|
| PRODUCING TRIACYLGLYCEROLS FROM GUM | 2009 |
|
RU2456338C2 |
| METHOD OF OBTAINING LOW-SATURATED OILS | 2013 |
|
RU2646057C2 |
| ENZYMATIC DEGUMMING | 2012 |
|
RU2600003C1 |
| METHODS FOR OIL REFINING | 2010 |
|
RU2573916C2 |
| COMPOSITION FOR ENZYMATIC REMOVAL OF SLIME FROM OILS | 2013 |
|
RU2582044C2 |
| PHOSPHOLIPASE C ENZYME PREPARATION BASED ON RECOMBINANT PRODUCER STRAIN BACILLUS MOJAVENSIS RCAM05968 BDV-1 PLC 6H FOR USE IN FOOD INDUSTRY AND METHOD FOR PRODUCTION THEREOF | 2023 |
|
RU2822431C1 |
| RECOMBINANT BACILLUS SUBTILIS BACTERIA STRAIN-PRODUCER OF SPECIFIC PHOSPHOLIPASE | 2012 |
|
RU2500811C1 |
| METHOD FOR REMOVAL OF RESINOUS SUBSTANCES FROM VEGETABLE OIL AND ITS REFINING | 2018 |
|
RU2772452C2 |
| METHOD FOR DEGUMMING TRIGLYCERIDE-CONTAINING COMPOSITIONS | 2015 |
|
RU2680688C2 |
| COMPOSITION FOR OIL ENZYMATIC DEGUMMING | 2015 |
|
RU2680690C2 |
