FIELD: chemistry.
SUBSTANCE: claimed invention relates to biochemistry and discloses method for oil refining. For method realisation water solution of acid is mixed with oil to obtain mixture, with pH 1-4, after which bases are added to said mixture with obtaining mixture with pH 6-8. As a result emulsion, which contains more than 60 vol % of water phase with drop size about 15-45 mcm. Recombinant phosphatidylinositol-specific phospholipase C (PI-PLC), which has amino acid sequence SEQ ID NO:8, is added into said emulsion. After realisation of described method stages inositoltriphosphate can be separated.
EFFECT: claimed invention makes it possible to effectively separate non-hydratable phospholipids (NHP) from oil due to application of novel recombinant phospholipase.
11 cl, 21 dwg, 39 tbl, 18 ex
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