FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology, specifically to the field of accurate transformation of plants, directed genome integration and protein expression in plants. Disclosed is a polynucleotide donor cassette for plant transformation, which contains a binding domain of site-specific nuclease, an analytic domain comprising a polynucleotide sequence with at least 99 % sequence identity with SEQ ID NO: 142, and a plasmid domain. Polynucleotide donor cassette is used to produce a transgenic cell for transformation of a plant.
EFFECT: inventions provide directed insertion of a universal polynucleotide donor cassette into the genome of a plant cell, fast screening of target sites and nucleases, as well as minimizing sequences that are difficult to amplify.
15 cl, 17 dwg, 8 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RAPID TARGETING ANALYSIS IN CROPS FOR DETERMINING DONOR INSERTION | 2014 |
|
RU2668819C2 |
| FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2665811C2 |
| CONSTRUCTED BY ENGINEERING TECHNIQUES PLATFORM (ETIP) FOR TRANSGENE EMBEDDING FOR GENETIC TARGETING AND TRAIT STACKING | 2013 |
|
RU2666916C2 |
| METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETING OF TRANSGENES | 2013 |
|
RU2650819C2 |
| FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2656159C2 |
| FLUORESCENCE ACTIVATED CELL SORTING (FACS) ENRICHMENT TO GENERATE PLANTS | 2013 |
|
RU2679510C2 |
| TARGETED MODIFICATION OF MALATE DEHYDROGENASE | 2013 |
|
RU2658437C2 |
| DNA DETECTION METHODS FOR SITE-SPECIFIC NUCLEASE ACTIVITY | 2013 |
|
RU2678001C2 |
| OPTIMAL SOYA LOCI | 2014 |
|
RU2719150C2 |
| FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2656158C2 |
