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2 481 396

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IPC

C12N5/00(2006-01-01)

G01N33/49(2006-01-01)

A61K35/12(2006-01-01)

(21) (22)

Application

2011137686/10, 2011-09-13

(24)

Start date

2011-09-13

(22)

Actual filing date

2011-09-13

(45)

Published

2013-05-10

(72)

Inventor

Jastrebov Anatolij PetrovichGrebnev Dmitrij Jur'EvichMaklakova Irina Jur'Evna

(73)

Holder

Gosudarstvennoe Bjudzhetnoe Obrazovatel'Noe Uchrezhdenie Vysshego Professional'Nogo Obrazovanija Gosudarstvennaja Meditsinskaja Akademija Ministerstva Zdravookhranenija I Sotsial'Nogo Razvitija Rossijskoj Federatsii" Vpo Ugma Minzdravsotsrazvitija Rossii)Obshchestvo S Ogranichennoj Otvetstvennost'Ju Kletochnykh Tekhnologij" Kletochnykh Tekhnologij")

METHOD TO EXTRACT HEMATOPOIETIC STEM CELLS BY METHOD OF IMMUNOMAGNETIC SEPARATION Russian patent published in 2013 - IPC C12N5/00 G01N33/49 A61K35/12

Abstract RU 2481396 C1

FIELD: biotechnologies.

SUBSTANCE: method consists in extraction of HSC due to removal of elements, which are not HSC, from a suspension containing different hematopoietic cells. A buffer for immunomagnetic separation (IMSB) is a separation cocktail (SC) of the following composition: PBS+2% solution of indispensable amino acids +2% FBS+1% solution of antibiotices per 100 ml of SC: penicillin 100 units/ml + streptomycin 100 mkg/m, incubation of a cell with particles for separation of Streptavidin Particles Plus - DM is carried out at - 20°C for 3 min. The invention may be used in cell therapy of different diseases.

EFFECT: method makes it possible to get substantial increase of viability of extracted HSC.

3 ex

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RU 2 481 396 C1

Authors

Jastrebov Anatolij Petrovich

Grebnev Dmitrij Jur'Evich

Maklakova Irina Jur'Evna

Dates

2013-05-10—Published

2011-09-13—Filed