IMMUNOLOGIC ANALYSES OF BOTULINUM TOXIN SEROTYPE A ACTIVITY Russian patent published in 2013 - IPC C07K16/12 A61K38/02 G01N33/543 

FIELD: chemistry.

SUBSTANCE: invention discloses composition for production of antibodies SNAP-25, capable of binding with epitope, which contains C-end (carboxylic) on residue P₁ of cleaved bond of cleavage site BoNT/A of SNAP-25 cleavage product, which contains carrier, flexible linker, containing at least three amino acids, and SNAP-25 antigen, for which amino acid sequence is given. Described are versions of antibodies α-SNAP-25 with said capability and their application in method of detecting BoNT/A activity with application of cells from stable cell line, sensitive to BoNT/A intoxication, and method of determining immunoresistance to BoNT/A in mammal with application of tested animal sample and cells of stable cell lime, whose cells are sensitive to BoNT/A intoxication.

EFFECT: application of invention ensures reliability, simplicity and makes it possible to exclude necessity of animal tests from botulinum toxin analyses.

15 cl, 11 dwg, 14 tbl, 12 ex