FIELD: biotechnology.
SUBSTANCE: method of production of induced pluripotent stem cells (IPS cells) from skin fibroblasts of patients with Down syndrome is proposed, which includes preliminary induction using lentiviral transfection with gene hTERT, further transfection of the resulting cells with three lentiviral constructs with genes Oct4, Sox2, Nanog, with subsequent cultivation for 14-21 days with valproic acid under conditions of low oxygen content 5% O2.
EFFECT: improvement of the method.
2 ex
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