FIELD: veterinary medicine.
SUBSTANCE: method comprises application of the vaccine into the cell culture of kidney of saiga, incubation for 1 hour at a temperature of 37±0.5°C, washing of the cell monolayer with the medium Igla MEM without serum, application of maintenance medium Igla with 2% cattle serum, incubation of the culture at a temperature of 37±0.5°C for 3-4 days, freezing of the culture at a temperature of -40°C, thawing, carrying out the 2nd passage in the above-mentioned manner, titrating of the obtained material and carrying out evaluation by the method of direct immunofluorescence on the 9-11th day.
EFFECT: invention may be used in research institutes and biofactories under the control of inactivated antirabic vaccine, namely in assessing its completeness of inactivation.
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