FIELD: veterinary virology. SUBSTANCE: method involves assay of antirabies virus-neutralizing antibodies by method of inhibition of fluorescence foci. Analyzed serum is mixed with an equal volume of attenuated strain of rabies virus TC-80 and incubated at 37 C for 90 min. Then reaction mixture is added to transplanted culture of saiga kidney cells, cell culture is fixed and antirabies immunoglobulins labeled with FITZ are applied. Reaction is estimated by inhibition of fluorescence foci in cell culture. Invention provides safety, sensitive and rapid method of assay of antirabies virus-neutralizing antibodies in vitro which shows correlation with titration results in experiments in white mice. EFFECT: improved method of assay, decreased cost. 1 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR TITRATION OF ANTIRABIC VIRUS-NEUTRALIZING ANTIBODIES | 2003 |
|
RU2254575C2 |
| METHOD FOR OBTAINING HYPERIMMUNE ANTIRABIC SERA | 2001 |
|
RU2196607C2 |
| METHOD OF PREPARING ANTIRABIC INACTIVATED CULTURAL VACCINE | 1994 |
|
RU2077338C1 |
| METHOD FOR DETERMINING SPECIFIC ACTIVITY OF ANTI-RABIES IMMUNOGLOBULIN ON CELL CULTURE USING ATOMIC FORCE MICROSCOPY | 2018 |
|
RU2688334C1 |
| METHOD OF CONTROL OF COMPLETENESS OF INACTIVATION OF ANTIRABIC INACTIVATED VACCINE | 2012 |
|
RU2492452C1 |
| VIRUS-VACCINE AGAINST RABIES FOR ORAL IMMUNIZATION OF CARNIVORE ANIMALS | 1999 |
|
RU2157700C2 |
| DIAGNOSTICUM AND TEST-SYSTEM FOR DETERMINING ACTIVITY OF ANTI-RABIES SERUMS AND PREPARATON OF HETEROLOGICAL ANTI-RABIES IMMUNOGLOBULIN IN VITRO BY DOT-IMMUNOANALYSIS METHOD | 2008 |
|
RU2360252C1 |
| TEST SET AND METHOD FOR DETECTING RIFT VALLEY FEVER VIRUS ANTIBODIES IN ANIMAL BLOOD SERUM | 1996 |
|
RU2112245C1 |
| METHOD FOR MANUFACTURING VIRAL ANTIRABIC VACCINE FOR ORAL IMMUNIZATION I WILD CARNIVORES | 2003 |
|
RU2250781C2 |
| ATTENUATED STRAIN OF CARNIVOROUS PLAGUE VIRUS PESTIS CARNIVORUM | 1996 |
|
RU2108385C1 |
