METHOD TO PREPARE BACTERIAL PREPARATION OF ACIDOPHILIC CULTURES Russian patent published in 2013 - IPC C12N1/20 A23C9/12 

FIELD: biotechnologies.

SUBSTANCE: method provides for preparation of a nutrient medium on the basis of a dry nutrient medium MEDIA TW-50 with application of a salt additive into it, such as dibasic sodium phosphate at the specified ratio. The nutrient medium is sterilised, cooled down to the specified temperature and neutralised, with 20% solution of caustic soda, with establishment of the specified pH value of the medium to produce the nutrient medium. The acidophilic culture of the strain 126 and yeast autolysate are introduced at the specified ratio into the produced nutrient medium with subsequent cultivation. At the same time in process of cultivation the acidity of the nutrient medium is maintained with staged deoxidisation by the 20% solution of the caustic soda. The bacterial mass is cooled and separated. The bacterial mass is mixed with the protective medium containing saccharose, gelatine and water, where additionally lactulose syrup is introduced, as a symbiotic protective factor, ascorbic acid, as an anti-enzyme agent, talc powder as a drying and sliding agent at the specified ratio. It is homogenised, poured, frozen and dried with a sublimation method.

EFFECT: invention makes it possible to increase survival rate of acidophilous bacteria under sublimation and storage of a preparation and to reduce hygroscopicity of a finished preparation.

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