FIELD: biotechnologies.
SUBSTANCE: test system for detection of bluetongue RNA virus of the 14-th serotype by means of a polymerase chain reaction in a real-time mode includes oligonucleotide primers and a DNA probe, which are complementary to sections of variable 2-nd segment, which have the following nucleotide composition (5'-3'): 14/2/qf GCT GTC GGG TAT AGG TAG GA; 14/2/z FAM - GTG AAC GTG GGG TCA TCT TCA C - BHQ1; 14/2/qr ACG TGT CCG ATG CTG CTA TC.
EFFECT: test system has increased specificity and sensitivity at reduction of the time required for performance of an operation relating to determination of a serotype of bluetongue virus.
2 tbl, 1 ex
Title |
Year |
Author |
Number |
TEST-SYSTEM FOR IDENTIFICATION OF RNA VIRUS OF BLUETONGUE BY RT-PCR METHOD IN REAL TIME MODE |
2012 |
- Panferova Agnesa Vladimirovna
- Tsybanov Sodnom Zham'Janovich
- Kolbasov Denis Vladimirovich
|
RU2510851C1 |
SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE C (SEROTYPES 6, 14 AND 21) BY RT-PCR METHOD |
2013 |
- Panferova Agnesa Vladimirovna
- Kol'Tsov Andrej Jur'Evich
- Tsybanov Sodnom Zham'Janovich
- Kolbasov Denis Vladimirovich
|
RU2529955C1 |
SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE B (SEROTYPES 3, 13 AND 16) BY RT-PCR METHOD |
2013 |
- Panferova Agnesa Vladimirovna
- Kol'Tsov Andrej Jur'Evich
- Tsybanov Sodnom Zham'Janovich
- Kolbasov Denis Vladimirovich
|
RU2528745C1 |
SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE A (SEROTYPES 4, 10, 11, 17, 20 AND 24) BY METHOD OF RT-PCR |
2013 |
- Panferova Agnesa Vladimirovna
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- Tsybanov Sodnom Zham'Janovich
- Kolbasov Denis Vladimirovich
|
RU2534358C1 |
SYNTHETIC OLIGONUCLEOTIDE PRIMERS-PROBE AND METHOD TO DETECT GENOMES OF 1ST, 4TH, 16TH SEROTYPES OF BLUETONGUE DISEASE VIRUS BY METHOD OF RT-PCR IN REAL-TIME MODE |
2011 |
- Panferova Agnesa Vladimirovna
- Tsybanov Sodnom Zhem'Janovich
- Guzalova Anna Grigor'Evna
- Lunitsin Andrej Vladimirovich
- Kolbasov Denis Vladimirovich
|
RU2481404C1 |
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2013 |
- Aronova Elena Vladimirovna
- Kalabekov Ismail Musaevich
- Tsybanov Sodnom Zham'Janovich
- Lunitsin Andrej Vladimirovich
|
RU2540142C2 |
KIT FOR DNA DETECTION OF BOVINE IMMUNODEFICIENCY PROVIRUS CONTAINING A PAIR OF SPECIFIC PRIMERS AND PROBE AND DIAGNOSTIC TECHNIQUE FOR CATTLE IMMUNODEFICIENCY VIRUS BY POLYMERASE CHAIN REACTION IN REAL TIME |
2015 |
- Krasnikova Ekaterina Sergeevna
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RU2595373C1 |
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2007 |
- Kalabekov Ismail Musaevich
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RU2378384C2 |
OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR DETECTING BLUETONGUE VIRUS GENOME BY POLYMERASE CHAIN REACTION IN ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS |
2007 |
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|
RU2385943C2 |
OLIGONUCLEOTIDE PRIMERS, DNA-PROBE AND METHOD FOR DETECTION OF VIRUS OF INFECTIOUS ANAEMIA IN HORSES BY METHOD OF PCR OR RT-PCR IN REAL TIME MODE |
2013 |
- Gerasimova Nadezhda Nikolaevna
- Panferova Agnesa Vladimirovna
- Kolbasova Ol'Ga L'Vovna
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|
RU2548799C1 |