TEST SYSTEM FOR DETECTION OF BLUETONGUE RNA VIRUS OF 14-TH SEROTYPE USING OT-PCR METHOD IN REAL-TIME MODE Russian patent published in 2013 - IPC C12Q1/68 

Abstract RU 2499054 C1

FIELD: biotechnologies.

SUBSTANCE: test system for detection of bluetongue RNA virus of the 14-th serotype by means of a polymerase chain reaction in a real-time mode includes oligonucleotide primers and a DNA probe, which are complementary to sections of variable 2-nd segment, which have the following nucleotide composition (5'-3'): 14/2/qf GCT GTC GGG TAT AGG TAG GA; 14/2/z FAM - GTG AAC GTG GGG TCA TCT TCA C - BHQ1; 14/2/qr ACG TGT CCG ATG CTG CTA TC.

EFFECT: test system has increased specificity and sensitivity at reduction of the time required for performance of an operation relating to determination of a serotype of bluetongue virus.

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RU 2 499 054 C1

Authors

Panferova Agnesa Vladimirovna

Kol'Tsov Andrej Jur'Evich

Tsybanov Sodnom Zham'Janovich

Kolbasov Denis Vladimirovich

Dates

2013-11-20Published

2012-10-11Filed