FIELD: biotechnologies.
SUBSTANCE: allergens are used in a diagnostics method of IgE-mediated allergy in vitro for wheat, which involves contact of a liquid sample of a mammal organism that is supposed to have IgE-mediated wheat allergy at least with one polypeptide with SEQ ID NO: 2 or 8, or with its fragment or variant having common epitopes for antibodies with the specified polypeptide and having the sequence identical to SEQ ID NO: 2 or 8, at least by 95%; and reveal of presence in the sample of IgE-antibodies that are specifically linked to the above polypeptide or polypeptides. Presence in the sample of such antibodies is a sign of IgE-mediated wheat allergy. Diagnostics set includes the above polypeptides and means for reveal of IgE connection to the above polypeptide, such as a hard substrate, for example nitrocellulose membrane or micromatrix containing a polypeptide linked to it.
EFFECT: invention allows effective diagnostics of IgE-mediated wheat allergy.
8 cl, 13 dwg, 9 tbl, 4 ex
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