FIELD: biotechnologies.
SUBSTANCE: treatment methods of inactivated virus or fragmented virus (versions) are proposed. Methods involve addition of virus preparation to sugar gradient and further centrifugation to obtain peak fraction. Then, peak fraction is extracted to obtain cleaned virus. With that, sugar gradient is obtained by continuous ultracentrifugation of the first and the second buffer sugar solution. Besides, the first buffer sugar solution includes the first physiological buffer, and the second buffer sugar solution includes the second physiological buffer that is the same or different from the first physiological buffer. As per one version, sugar concentration in the first buffer solution is equivalent to saccharose concentration of 35 to 50 wt %, and sugar concentration in the second buffer solution is equivalent to saccharose concentration of 50 to 65 wt %. Besides, the second buffer sugar solution has higher density in comparison to that of the first one. As per the other method version, density of the first buffer sugar solution is 1.15 kg/l to 1.23 kg/l, and density of the second one is 1.23 kg/l to 1.32 kg/l.
EFFECT: methods result in increased virus output and minimisation of virus aggregation in saccharose peak fraction.
26 cl, 1 dwg, 9 tbl, 10 ex
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Authors
Dates
2014-01-10—Published
2008-04-30—Filed