METHOD FOR INTRODUCING siRNA INTO CELLS BY PHOTOCHEMICAL INTERNALISATION Russian patent published in 2014 - IPC C12N15/87 A61K41/00 

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology, more specifically to a method for introducing an siRNA molecule into the cytosol of a cell, and can be used in medicine. The method involves contacting said cell with an siRNA molecule, a carrier and a photosensiting agent, and irradiating the cell with light of a wavelength effective to activate the photosensitising agent. The carrier comprises a cationic polyamine such as a lipopolyamine in a non-liposomal formulation, branched polyethyleneimine (PEI), a betacyclodextrin amine polymer, a PAMAM dendrimer molecule, and a cationic peptide such as polyarginine or L- or D-arginine copolymers. The method is used to inhibit the target gene expression, to obtain a cell or a cell population containing the siRNA molecules, as well as to treat or to prevent a disease where the inhibition of one or more genes may be effective, including to treat a malignant growth.

EFFECT: invention enables the PCI-mediated site-specific siRNA delivery into the cytosol of a cell.

27 cl, 15 dwg, 1 tbl, 13 ex