FIELD: biotechnology.
SUBSTANCE: described is a recombinant attenuated strain of vaccinia virus with broken virulence genes ΔA56RΔB8RΔJ2RΔC3LΔN1L (1421ABJCN) based on cloned LIVP strain of vaccinia virus. Technical result of proposed invention consists in creation using five plasmids integration of highly attenuated recombinant vaccinia virus strain for producing safer live culture of attenuated smallpox vaccine and other orthopoxviruses, pathogenic for humans. Said technical result is achieved by obtaining recombinant strain L-IVP 1421ABJCN vaccinia virus with broken virulence genes A56R, B8R, J2R, C3L, NIL, intended for producing live culture of attenuated smallpox virus vaccine and other orthopoxviruses.
EFFECT: invention can be used in medicine, biotechnology, particularly genetic engineering for producing live culture of attenuated smallpox vaccine and other orthopoxviruses, pathogenic for humans.
1 cl, 9 dwg, 5 tbl, 12 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STAIN OF VACΔ6 VACCINIA VIRUS WITH BROKEN GENES OF VIRALENCE C3L, N1L, J2R, A35R, A56R, B8R FOR THE PRODUCTION OF LIVE CULTURAL ATTENUATED VACCINE AGAINST NATURAL SMALLPOX AND OTHER ORTHOPOXVIRAL HUMAN INFECTIONS | 2016 |
|
RU2621868C1 |
| LIVE ATTENUATED CULTURE VACCINE FOR PREVENTION OF SMALLPOX AND OTHER ORTHOPOXVIRUS INFECTIONS BASED ON SMALLPOX VACCINE VIRUS AND METHODS FOR ITS PREPARATION AND APPLICATION | 2022 |
|
RU2781070C1 |
| ANTI-TUMOUR AGENT BASED ON RECOMBINANT VACCINIA VIRUS STRAIN AND METHOD FOR PRODUCTION THEREOF | 2019 |
|
RU2730657C1 |
| RECOMBINANT STRAIN VV-GMCSF-Lact OF VACCINIA VIRUS, HAVING ONCOLYTIC ACTIVITY AND PRODUCING GRANULOCYTIC-MACROPHAGAL COLONY-STIMULATING FACTOR AND ONCOTOXIC LACTAPTIN PROTEIN | 2015 |
|
RU2604187C1 |
| RECOMBINANT ONCOLYTIC STRAIN OF L-IVP_ONCOQ VACCINIA VIRUS, CONTAINING GENES ENCODING GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR AND POLYEPITOPE IMMUNOGEN CONSISTING OF ANTIGEN EPITOPES, OVEREXPRESSING TUMOR CELLS IN OVARIAN CANCER | 2017 |
|
RU2678003C1 |
| RECOMBINANT ONCOLYTIC STRAIN OF THE VACCINIA VIRUS L-IVP_ONCOB, CONTAINING GENES, ENCODING GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR AND POLYEPITOPE IMMUNOGEN, CONSISTING OF EPITOPES OF ANTIGENS, HYPER EXPRESSING BY TUMOR CELLS IN BREAST CANCER | 2017 |
|
RU2678056C1 |
| RECOMBINANT ONCOLYTIC STRAIN OF L-IVP_ONCOM VACCINIA VIRUS, CONTAINING GENES, ENCODING GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR AND POLYEPITOPE IMMUNOGEN CONSISTING OF ANTIGEN EPITOPES, OVEREXPRESSING TUMOR CELLS IN MELANOMA | 2017 |
|
RU2678060C1 |
| RECOMBINANT VARIOLOVACCINE VIRUS STRAIN VV-NS1-dGF PRODUCING NS1 PARVOVIRUS H-1 PROTEIN AND HAVING ONCOLYTIC ACTIVITY ON HUMAN GLIOBLASTOMA | 2018 |
|
RU2692628C1 |
| RECOMBINANT STRAIN VV-GMCSF-S1/3 OF VACCINIA VIRUS, PRODUCING SECRETED GRANULOCYTE-MACROPHAGE COLONY-STIMULATING HUMAN FACTOR | 2013 |
|
RU2565544C2 |
| RECOMBINANT PLASMID DNA pGEM-Puro-DS-Apo COMPRISING SYNTHETIC APOPTIN GENE FLANKED BY SEQUENCES OF VACCINIA VIRUS GENOME OF ISLAND C10L-C12L, AND RECOMBINANT STRAIN VVdGF-ApoS24/2 OF VACCINIA VIRUS PRODUCING APOPTIN | 2012 |
|
RU2492238C1 |
