FIELD: biotechnologies.
SUBSTANCE: method involves transfectant cells of HeLa line obtained by introduction to their chromosome of plasmid vector pBI/neo/X (X - any eukaryote transcription factor) containing minimum promoter of human cytomegalovirus, gene of green light-harvesting protein, sequence of nucleotides coding the fixation point of transcription factor, and neomycin resistance gene. Activity of transcription factor is determined by measurement of vital fluorescence intensity of the obtained cell culture in presence of test substance in comparison to intact cell culture.
EFFECT: invention allows quick and high-sensitive determination of activity of eukaryote transcription factors.
2 dwg, 1 ex
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