METHOD OF PRODUCTION in vitro OF POTATO FORMS RESISTANT TO PHYTOPHTHORA AND ALTERNARIA SPOT CAUSATIVE AGENTS Russian patent published in 2014 - IPC A01H1/00 A01H4/00 A01H5/00 

FIELD: biotechnology.

SUBSTANCE: leaf explants isolated from the thirty-day aseptic plants of initial varieties grown in 1 l containers, are placed in Petri dishes with the liquid medium of a certain composition and preliminary incubated, co-cultivated and cultivated on nutrient media of a certain composition. When appearance of regenerants of the size at least 10 mm they are cut and transferred to the nutrient media of a certain composition. The first stage of selection of forms resistant to Phytophthora and Alternaria spot causative agents is carried out, by culturing the cut regenerants on MS medium with 50 ml/L kanamycin sulfate for 30-45 days at 22-25°C and illumination of 6000-10000 lux at 16-hour photoperiod followed by selection of rooted regenerants of potatoes with green leaves and stems. The second stage of selection of forms resistant to Phytophthora and Alternaria spot causative agents is carried out, including checking of rooted plants by PCR method for the presence of target DNA and reproduction of regenerants with confirmed PCR target DNA insert by micrografting.

EFFECT: use of claimed method enables to obtain potato forms resistant to Phytophthora and Alternaria spot causative agents.

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