METHOD OF PRODUCING NANOMATERIAL BASED ON RECOMBINANT ARCHAEAL Halobacterium salinarum FLAGELLA Russian patent published in 2014 - IPC C12N1/20 C12N15/09 C12N15/63 B82Y5/00 B82Y30/00 

FIELD: chemistry.

SUBSTANCE: invention concerns biotechnology and nanotechnology. The method includes transforming archaeal cells with a recombinant plasmid, growing cells, selecting flagella and modifying the surface of the flagella. The plasmid structure contains recombinant genes for synthesis of flagellins A1 and A2 which form flagella, wherein the sequence of flagellin A1 or flagellin A2 or sequences of flagellin A and flagellin A2 contain at least one peptide insert for selective binding of metal ions or nanoparticles. The point of the peptide insert in flagellin A1 is defined in the region between first and second glycosylation sites located between position 86 and position 96 of SEQ ID NO:2, and the point of the peptide insert in flagellin A2 is defined in the region between first and second glycosylation sites located between position 82 and position 92 of SEQ ID NO:3, where the length of the peptide insert is 5 to 60 amino acids. The method includes selecting archaeal flagella containing peptide inserts for non-covalent bonding with metal ions, performing fragmentation of flagella into fragments and modifying the surface of flagella by binding peptide inserts with metal ions and oxidising metals, washing, drying and packing the obtained nano-structured material.

EFFECT: method enables to obtain a coating for forming active surfaces on flexible and solid substrates or capsules using archaeal flagella, which enable non-covalent bonding of a wide range of substances such as metal ions, metal nanoparticles, semiconductors and other ligands.

6 cl, 11 dwg, 1 tbl, 12 ex