BIOLOGICAL SENSOR AND METHOD OF CREATION OF BIOLOGICAL SENSORS Russian patent published in 2014 - IPC G01N33/53 C12Q1/00 

FIELD: biotechnology.

SUBSTANCE: biological sensor is described, as well as the method of creation of a biological sensor using thin films based on grapheme, graphene oxide or single-walled or multi-walled carbon nanotubes. The biological sensor comprises a substrate, a metal film on the surface of which the intermediate bonding layer is applied, made of a thin film of graphene or a thin film of graphene oxide or a thin film of carbon nanotubes. On the surface of the intermediate bonding layer the biospecific layer is adsorbed conformally and uniformly. The biospecific layer can be used as a layer of molecules of the binding partner of the analyte or a layer of a complex of biological molecules capable of interacting chemically with the molecules of the binding partner and forming a complex with them. Also the biospecific layer can be used as the hydrogel layer, on which the binding partner molecule and/or complex of binding partner molecules and biological molecules is deposited, which can form a chemical bond with the binding partner molecules. The described method of obtaining the biological sensor comprises the stages of applying a metal film, an intermediate bonding layer and a biospecific layer.

EFFECT: high sensitivity of the biosensor in combination with high biospecificity, expanding the range of application of the device, protection of the metallic film from the effects of the environment, the ability to detect large biological objects.

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