FIELD: medicine, pharmaceutics.
SUBSTANCE: present invention concerns a method for propagation and differentiation of pluripotent cells. The described method involves the stages as follows: culturing the pluripotent cells and processing the pluripotent cells with GSK-3B inhibitor, wherein the above inhibitor represents 3-[1-(2-hydroxyethyl)-1H-indol-3-yl]-4-(1-pyridin-3-yl-1H-indol-3-yl)-pyrrole-2,5-dione.
EFFECT: presented invention enables preparing an adequate amount of the cell material with maintaining an ability for differentiation to be further used for clinical applications.
10 cl, 15 dwg, 11 tbl, 11 ex
Authors
Dates
2014-09-20—Published
2009-04-22—Filed