FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to methods for differentiating pluripotent stem cells, which are cells of the human embryonic stem cell line H1, cells of the human embryonic stem cell line H7, human embryonic stem cell lines H9, cells of the human embryonic stem cell line SA002 or mutant human embryonic stem cell lines BG01v, into cells expressing markers characteristic of the line of the formed endoderm. Method comprises treating said pluripotent cells with a medium containing GDF-8 and 6-[(2-{[4-(2,4-dichlorophenyl)-5-(4-methyl-1H-imidazol-2-yl)pyrimidin-2-yl]amino}ethyl)amino]pyridine-3-cabonitrile, or a medium containing GDF-8, and cyclic aniline-pyridinotriazine selected from the group consisting of 5-chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1~3,7~.1~9,13~.1~14,18~]tritriacont-3(33),4,6,9(32),10,12,14(31),15,17-nonaene-23-one, 14-prop-2-en-1-yl-3,5,7,14,17,23,27-heptaazatetracyclo[19.3.1.1~2,6~.1~8,12~]-heptacose-1(25),2(27),3,5,8(26),9,11,21,23-nonaene-16-one and 14-methyl-3,5,7,14,18,24,28-heptaazatetracyclo[20.3.1.1~2,6~.1~8,12~]octacosa-1(26),2(28),3,5,8(27),9,11,22,24-nonaene-17-one, or GDF-8 and cyclic aniline-pyridinotriazine selected from the group consisting of 5-chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1~3,7~.1~9,13~.1~14,18~]tritriacont-3(33),4,6,9(32),10,12,14(31),15,17-nonaene-23-one, 14-prop-2-en-1-yl-3,5,7,14,17,23,27-heptaazatetracyclo[19.3.1.1~2,6~.1~8,12~]heptacose-1(25),2(27),3,5,8(26),9,11,21,23-nonaene-16-one and 14-methyl-3,5,7,14,18,24,28-heptaazatetracyclo[20.3.1.1~2,6~.1~8,12~]octacosa-1(26),2(28),3,5,8(27),9,11,22,24-nonaene-17-one.
EFFECT: invention makes it possible to improve the differentiation process.
18 cl, 126 dwg, 19 tbl, 24 ex
