FIELD: medicine.
SUBSTANCE: invention refers to medicine, biotechnology and cell technologies. A method for differentiating pluripotent stem cells presenting a human cell line in cells expressing markers specific for a formed endoderm line involves preparing the pluripotent stem cells in a medium differing by the fact that it is free from activin A and contains GDF-8 for the period of time adequate to differentiate the pluripotent stem cells in the cells expressing the markers specific for a formed endoderm line.
EFFECT: invention can be used in medicine for transplantation applications.
14 cl, 19 tbl, 27 dwg, 24 ex
