METHOD FOR EXPERIMENTAL ASSESSMENT OF MILLIMETRE-RANGE ELECTROMAGNETIC WAVES (EHF) EFFECT Russian patent published in 2014 - IPC A61N5/02 A61N5/10 A61B17/04 A61K31/675 A61P35/00 G01N33/48 G09B23/28 

FIELD: medicine.

SUBSTANCE: invention refers to experimental medicine and immunology, and can be used for the assessment of the millimetre-range electromagnetic wave (EHF) effect within a three-component model of cytostatic exposure. That is ensured by a thymus exposure in the group of animals to the millimetre-range electromagnetic wave (EHF) effect at wavelength 5.6 mm for 2 weeks every 1-2 days. A single exposure of the physical factor makes 1-2 minutes for 2 weeks every 1-2 days. That is followed by simulating a surgical intervention by opening and closing a peritoneum. On the seventh postoperative day, the animals are exposed to three-fold fractionated external gamma exposure in a single dose of 2.5 Sv every single day. Thereafter, cyclophosphan is administered intraperitoneally in a dose of 4 mg/100 g of animal's body weight. On the 14th day after the cytostatic is administered, the animals are killed to examine their blood and immune competent organs. That is combined with determining a thymus cellularity (TC) in 10⁶cell./100 mg of its weight, a functional lymphocyte activity in a nitroblue tetrazolium test (NTT) in units, a spleen antibody-forming cell (AFC) count according to N.K.Erne in cells/"чП", apoptosis in an annexin test (AT), %, blood serum circulating immune complex (CIC) in units. That is followed by calculating a physical factor effect index (FI) by formula: $$FI=\frac{AT\times NTT\times TC\times 100}{CIC\times AFC}.$$ If the FI is less than 48, the presence of the immunomodulatory effect of the EHF-exposure is stated.

EFFECT: method provides the objective assessment of the millimetre-range electromagnetic wave (EHF) effect within the three-component model of the cytostatic exposure.

1 tbl, 3 ex