METHOD OF DETECTING STEM CANCER CELLS Russian patent published in 2014 - IPC C12Q1/68 C12Q1/24 

FIELD: biotechnology.

SUBSTANCE: method of detecting stem cancer cells is provided, based on incubation of cell samples with fluorescence dyes and subsequent identification of cancer cells in the ultraviolet light, where during the incubation of cell samples, which is carried out with the fluorescent dye, incorporated covalently into double-stranded DNA fragments, the internalization of extracellular exogenous fragments of double-stranded DNA into the intracellular space of stem cells included in the composition of the sample cells, is provided by incubation of cell samples in the solution of the preparation of the fragmented double-stranded DNA at a ratio of 0.5-1 mcg DNA per 1000000 cells in suspension or on a section of tissue for 60-120 minutes, and identification of cancer cells as stem is carried out in ultraviolet light with a wavelength corresponding to the absorption maximum of the fluorochrome inserted into the molecules of fragments of the double-stranded DNA, and the double-stranded DNA fragments are used as DNA fragments of Alu human repeat, enzymatically labelled by precursor comprising covalently sewn fluorochrome dye, or labelled by direct chemical introduction of fluorochrome.

EFFECT: due to increasing the efficiency of detecting stem cancer cells in the initiating condition the invention can be used in medicine.

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