BIOTRANSPLANT FOR RECOVERY OF BONE TISSUE VOLUME IN CASE OF DEGENERATIVE DISEASES AND TRAUMATIC INJURIES OF BONES AND METHOD OF OBTAINING THEREOF Russian patent published in 2014 - IPC A61L27/24 A61K35/28 A61P19/10 

FIELD: medicine.

SUBSTANCE: invention relates to the field of medicine, namely to cell transplantology for the regeneration of a bone tissue in surgical treatment of destructive, degenerative-dystrophic, traumatic or congenital affections of the bone tissue. A biotransplant contains a mixture of autologous multipotent mesenchymal stromal cells, cultivated in standard conditions, and multipotent mesenchymal stromal cells, differentiated in the osteogenic direction, taken in a weight ratio of 1:1 and in quantity of 10⁷ cells per 1 g of the weight of a matrix-carrier, which has in the basis of its structure a collagen-mineral complex, identical in the composition to natural bone material. A method of the biotransplant obtaining is based on the application of the culture medium AdvanceSTEM^TM/Antibiotic/Antimycotic Solution 100x with an addition of 10% of autologous serum of the patient's blood.

EFFECT: technical result of the invention consists in the provision of active angiogenesis with the formation of small capillaries, medium and large mature vessels in a young bone tissue together with the preservation of its osteoinductive properties; elimination of a risk of the immune response and an infection transmission caused by the application of embryonic calf serum; reduction of terms of the bone tissue recovery and its vascularisation.

2 cl, 2 dwg, 2 tbl