COMPOSITIONS AND METHODS USED FOR ASSESSING CYTOTOXICITY OF SINGLE CELLS Russian patent published in 2014 - IPC C12Q1/68 

FIELD: pharmacology.

SUBSTANCE: method for assessing the cytolytic activity of single effector cells with defining a profile of the secreted products and using microcell matrixes; it involves monitoring of an infected target cell lysis with using the effector cells in the microwells, wherein the microwells contain one effector cell and at least one infected target cell per a microwell on the average; wherein the monitoring procedure involves the stage of detecting the above infected target cell lysis with using the effector cells by means of a fluorescent indicator, thereby defining a profile of one or more secreted products.

EFFECT: method can be used in medicine for large-scale screening for defining the profile of a great number of single cells in microarrays.

20 cl, 10 dwg, 7 ex