FIELD: pharmacology.
SUBSTANCE: there is a suspension of live mononuclear cells of peripheral blood of an individual, applied onto a plastic dish, which comprises at least one microwell in a matrix of microwells. At the same time at least one microwell in the specified matrix of microwells comprises one cell in the volume of at least one nanolitre. The matrix of microwells is put in contact with a substrate, besides, the substrate is previously treated by at least two agents for detection, and each detection agent is bound with a secreted cytokine of the specified cell. They measure the level of each specified secreted cytokine on the specified substrate, at the same time the level corresponds to quantity of the specified secreted cytokine of the specified single cell. They determine speed of secretion of each specified secreted cytokine from the specified cell, thus determining characteristics of the specified secreted cytokines. They measure dynamic change of the specified characteristics of secreted cytokines.
EFFECT: development of immunological characteristics of diseases.
41 cl, 4 tbl, 21 dwg, 4 ex
