METHOD OF PRODUCTION OF BIOLOGICALLY ACTIVE SUBSTANCE-SELENOCYSTINE Russian patent published in 2014 - IPC A61K33/04 A61K31/198 A23L1/304 A23L1/305 C12P13/12 

FIELD: biotechnology.

SUBSTANCE: method of production of selenocystine consists in that 1 molar solution of sodium diselenide is prepared by adding 4.5 g of elemental selenium to 10 ml of 1 molar solution of sodium hydroxide (NaOH) in a three-necked flask. Then, 15 ml of distilled water is added and the solution is stirred using a magnetic stirrer. The separately prepared solution of natrium borane (NaBH₄) by dissolving it in 25 ml of distilled water is added as drops to the three-necked flask with a suspension of elemental selenium to total decolorization of the solution, and another 4.5 g of elemental selenium are additionally added. 5 g β-chloro-l-alanine are dissolved in distilled water, and the solution is adjusted to pH 9 by adding 1M sodium hydroxide solution. Within 30 minutes the solution of β-chloro-l-alanine is added to the solution of sodium diselenide, located in the three-necked flask and stirred for 12-16 hours at a temperature of +37°C. Then, muriatic acid is added to the resulting solution dropwise to pH 2 and the air purging is carried out for 2-3 hours. The solution is filtered and adjusted with 10 M sodium hydroxide solution to pH 6-6.5. The solution is cooled to +5°C.

EFFECT: increase in the yield ratio and improved purity.

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