METHOD FOR DETECTING WIDE DYNAMIC RANGE ANALYTE CONCENTRATION Russian patent published in 2014 - IPC G01N33/53 

FIELD: medicine.

SUBSTANCE: invention refers to immunoassay methods aiming at detecting or measuring desired analytes in samples and may be used in laboratory or clinical practice for detecting antibodies, proteins, hormones, dosage forms in biological samples in the wide concentration range. Substance of the method consists in immobilising the first specific component having high affinity to the desired analyte on a solid carrier on the first microscopic zone, immobilising the first specific component having low affinity to the desired analyte on the second microscopic zone; introducing the analyte and the second specific binding component tagged with the first detected label and having high affinity to the analyte onto the microscopic zones simultaneously; incubating the first mixture to prepare the first specific complex tagged with the first detected label; adding the third specific binding component tagged with the second detected label and having low affinity to the desired analyte to the first mixture, with the second and third binding components having identical epitope specificity; incubating the second mixture to prepare the second specific complex tagged with the second detected label; removing the unbound reaction products and detecting signals generated by the labels bound to the first and second specific complexes in the first and second microscopic zones; measuring the analyte concentration by comparing the measured signals to a calibration curve plotted for the known values of the analyte concentration.

EFFECT: using the method enables more accurate determination of the analyte concentrations.

6 cl, 3 ex, 8 dwg