ENZYMATIC AGENT BASED ON IMMOBILISED BUTYRYLCHOLIN ESTERASE AND METHOD FOR ITS PREPARATION Russian patent published in 2015 - IPC C12N11/04 C12N9/18 

FIELD: biotechnologies.

SUBSTANCE: invention proposes a preparation method of an enzymatic agent based on immobilised butyrylcholin esterase and an enzymatic agent for determination of carbamates and organic phosphorus compounds. Starch or gelatine gel is prepared in a phosphate buffer pH 7.9-8.0. The gel is cooled down to 31-35°C. Then, it is mixed with the buffer solution of butyrylcholin esterase and a buffer solution of an indicator of thiolic groups of 5,5'-dithio-bis(2-nitrobenzoic acid) with a concentration of 0.56 M. The obtained mixture is dosed to a hydrophobic substrate and dried at the temperature of 8°C. The inventions allow simplifying an enzyme immobilisation procedure, increasing storage time of an enzymatic agent without any loss of its activity during more than one year.

EFFECT: agent has high sensitivity to action of organic phosphorus compounds and carbamates.

2 cl, 7 dwg, 7 ex