FIELD: biotechnology.
SUBSTANCE: described is a method for detecting RNA of the measles virus by real-time RT-PCR. It provides for extraction of RNA from biological samples, followed by a two-step reaction—reverse transcription and polymerase chain reaction for the target RNA fragments of the virus genome. Method involves using a set of oligonucleotide primers and a corresponding fluorescent labeled probe.
EFFECT: wider range of products used to detect the measles virus.
1 cl, 1 dwg, 4 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD OF DETECTING NIPAH VIRUS USING REAL-TIME RT-PCR | 2022 |
|
RU2816270C2 |
| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
RU2744198C1 |
| METHOD FOR DETECTING MODIFIED POLIOVIRUS VACCINE TYPE 2 (nOPV2) RNA BY REAL-TIME RT-PCR | 2022 |
|
RU2795703C1 |
| METHOD OF DETECTING MONKEY POX VIRUS OF MONKEYPOX TYPE BY REAL TIME PCR (MPX AMP PS) | 2023 |
|
RU2803898C1 |
| METHOD FOR TICK-BORNE ENCEPHALITIS VIRUS DETECTION BY REAL-TIME RT-PCR METHOD | 2019 |
|
RU2744187C1 |
| METHOD OF DETECTING NIPAH VIRUS BY METHOD BASED ON USE OF DEOXYRIBOZYME 10-23 | 2023 |
|
RU2816271C1 |
| METHOD FOR DETECTING GENOME OF PARAINFLUENZA VIRUS OF TYPE 3 IN BOVINE ANIMALS | 2018 |
|
RU2696069C2 |
| METHOD FOR DETECTING RNA OF SCHMALLENBERG DISEASE VIRUS IN FARM ANIMALS | 2018 |
|
RU2696306C1 |
