FIELD: biotechnology.
SUBSTANCE: described is a method for detecting Hendra henipavirus RNA by real-time RT-PCR. Method includes extraction of RNA from biological samples, followed by a two-step reverse transcription reaction and a polymerase chain reaction for the target RNA fragments of the viral genome using a set of oligonucleotide primers and a corresponding fluorescent labelled probe, complementary to a section of the gene coding nucleocapsid protein N of Hendra virus.
EFFECT: development of a highly sensitive method for identifying Hendra virus RNA in biological samples.
1 cl, 1 dwg, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTING NIPAH VIRUS USING REAL-TIME RT-PCR | 2022 |
|
RU2816270C2 |
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| METHOD FOR DETECTING MODIFIED POLIOVIRUS VACCINE TYPE 2 (nOPV2) RNA BY REAL-TIME RT-PCR | 2022 |
|
RU2795703C1 |
| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
RU2744198C1 |
| METHOD OF DETECTING MONKEY POX VIRUS OF MONKEYPOX TYPE BY REAL TIME PCR (MPX AMP PS) | 2023 |
|
RU2803898C1 |
| METHOD FOR TICK-BORNE ENCEPHALITIS VIRUS DETECTION BY REAL-TIME RT-PCR METHOD | 2019 |
|
RU2744187C1 |
| METHOD OF DETECTING NIPAH VIRUS BY METHOD BASED ON USE OF DEOXYRIBOZYME 10-23 | 2023 |
|
RU2816271C1 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN BIOLOGICAL MATERIAL WITH A LOW VIRAL LOAD BASED ON TWO-STAGE PCR WITH DETECTION BY THREE TARGETS IN REAL TIME | 2020 |
|
RU2763173C1 |
| METHOD FOR DETECTING RNA OF SCHMALLENBERG DISEASE VIRUS IN FARM ANIMALS | 2018 |
|
RU2696306C1 |
