FIELD: chemistry.
SUBSTANCE: group of inventions can be used to detect a salmon yersiniosis agent - Yersinia ruckeri - in samples for diagnosing fish diseases in veterinary. The method includes performing a polymerase chain reaction to detect corresponding nucleic acid fragments using primers, having the following structure: 5'CTAACACTACAGGTTATCGCCTCTG3' (Yr-rA-1), 5'GGCTCATCATACGAGCGGCAAGTCC3' (Yr-rA-2), 5'CTTGTTTGACGATATTCGCTTTGGC3' (Yr-gA-1), 5'CTGGGGGAACCGGGAAGTAACC3' (Yr-gA-2), 5'GCGAAAGCTTGCGTGAGCTGTCG3' (Yr-gB-1), 5'GGAAACCATCATTCCACTGCAAG3' (Yr-gB-2). Presence of Yersinia ruckeri DNA in the sample is determined from a specific fragment with a size of 294 bp for the Yr-rA-1,2 pair; with a size of 154 bp for the Yr-gA-1,2 pair; with a size of 225 bp for the Yr-gB-1,2 pair.
EFFECT: invention enables to diagnose salmon yersiniosis with high specificity.
2 cl, 3 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR SERUM DIAGNOSIS OF SALMON FISH YERSINIOSIS CAUSED BY Yersinia ruckeri BY ENZYME IMMUNOASSAY AND DIAGNOSTIC SET FOR IMPLEMENTING METHOD | 2015 |
|
RU2595883C1 |
| METHOD TO DIFFERENTIATE STRAINS OF PLAGUE AGENT OF PRIMARY SUBTYPE OF MEDIEVAL AND ANTIQUE BIOVARS BY METHOD OF POLYMERASE CHAIN REACTION | 2012 |
|
RU2496882C2 |
| METHOD OF GENETIC TYPING OF YERSINIA PESTIS AND YERSINIA PSEUDOTUBERCULOSIS BASED ON THE ANALYSIS OF 13 VNTR LOCUSES IN MULTIPLEX PCR REACTION | 2017 |
|
RU2644236C1 |
| METHOD DETECTION AND EVALUATION OF BIOTYPE, SEROGROUP AND TOXIGENICITY OF CHOLERA AGENT AND RELATED KIT | 2007 |
|
RU2360972C1 |
| SET AND METHOD FOR ACCELERATED PLAGUE MICROBE IDENTIFICATION AND SIMULTANEOUS DIFFERENTIATION OF VIRULENT AND AVIRULENT STRAINS OF YPESTIS BY PLASMID PROFILING THEREOF | 2011 |
|
RU2473701C1 |
| METHOD OF IDENTIFICATION AND INTRAGENERIC DIFFERENTIATION OF STRAINS OF SPECIES YERSINIA PESTIS | 2009 |
|
RU2404251C1 |
| METHOD OF DIFFERENTIATING TYPICAL AND ATYPICAL STRAINS Yersinia pestis OF MEDIEVAL BIOVAR BY PCR METHOD WITH HYBRIDISATION-FLUORESCENT RECORDING RESULTS | 2014 |
|
RU2550257C2 |
| METHOD FOR DIFFERENTIATING YERSINIA PESTIS STRAINS OF VARIOUS SUBSPECIES AND BIOVARS BY POLYMERASE CHAIN REACTION AND MULTILOCUS SEQUENCE TYPING | 2011 |
|
RU2471872C1 |
| METHOD FOR DETECTING ANTIBIOTIC-RESISTANCE GENES IN PLAGUE AGENT STRAINS BY POLYMERASE CHAIN REACTION | 2011 |
|
RU2456348C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENTLY LABELLED PROBE FOR IDENTIFICATION OF Burkholderia pseudomallei | 2014 |
|
RU2556810C1 |
