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2 799 415

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IPC

C12N1/00(2006-01-01)

C12N1/20(2006-01-01)

C12Q1/00(2006-01-01)

C12Q1/04(2006-01-01)

C12Q1/6806(2018-01-01)

(21) (22)

Application

2022120285, 2022-07-22

(24)

Start date

2022-07-22

(22)

Actual filing date

2022-07-22

(45)

Published

2023-07-05

(72)

Inventor

Nikiforov Konstantin AlekseevichOglodin Evgenij GennadevichKukleva Lyubov MikhajlovnaEroshenko Galina AleksandrovnaKutyrev Vladimir Viktorovich

(73)

Holder

Federalnoe Kazennoe Uchrezhdenie Nauki Nauchno-Issledovatelskij Protivochumnyj Institut Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka Rossijskij Protivochumnyj Institut Rospotrebnadzora)

METHOD OF DETERMINING THE PHYLOGENETIC AFFILIATION OF STRAINS OF YERSINIA PESTIS OF THE MAIN SUBSPECIES BY ALLELE-SPECIFIC PCR IN REAL TIME Russian patent published in 2023 - IPC C12N1/00 C12N1/20 C12Q1/00 C12Q1/04 C12Q1/6806

Abstract RU 2799415 C1

FIELD: biotechnology.

SUBSTANCE: method of determining the phylogenetic affiliation of Y. pestis strains of the main subspecies by real-time allele-specific PCRin UBSTANCE is described. The method involves isolating of the DNA of the strain, performing RT-PCR on 14 DNA targets with two direct primer variants, using the appropriate primers and probes having the following sequences of SEQ ID NO: 1-14 on target DNA "0.ANT1", "0.ANT2", "0.ANT3", "0.ANT5", "3.ANT", "4.ANT", "2.MED0", "2 .MED1", "2.MED2", "2.MED3", "2.MED4", "1.ORI1", "1.ORI2", "1.ORI3", and differentiation of strains according to their belonging to phylogenetic branches according to the presence/absence of fluorescence signals with the indicated primers.

EFFECT: invention expands the arsenal of agents that provide fast and reliable identification of Y. pestis strains by their belonging to the phylogenetic branches of the main subspecies 0.ANT1, 0.ANT2, 0.ANT3, 0.ANT5, 3.ANT, 4.ANT, 2.MED1, 2.MED2, 2.MED3, 2.MED4, 1.ORI1, 1.ORI2, 1.ORI3.

1 cl, 1 tbl, 3 ex

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RU 2 799 415 C1

Authors

Nikiforov Konstantin Alekseevich

Oglodin Evgenij Gennadevich

Kukleva Lyubov Mikhajlovna

Eroshenko Galina Aleksandrovna

Kutyrev Vladimir Viktorovich

Dates

2023-07-05—Published

2022-07-22—Filed