METHOD OF ASSESSMENT OF GENETIC FULL-VALUE OF SPERM Russian patent published in 2015 - IPC G01N33/48 

Abstract RU 2568845 C1

FIELD: veterinary medicine.

SUBSTANCE: method comprises adding to the ejaculate samples in vitro in a medium for dilution of hydrogen peroxide at a concentration of 0.5-1.0 mM, followed by the identification and determining in the sperm of primary morphological signs of apoptosis, nicking and DNA fragmentation, the intensity of lipid peroxidation. And in detection of the low percentage of apoptotic dying sperm up to 10-15% of bright enough and expressed halo glow, increasing the content of TBA-reactive products of not more than 30-40% relative to control, the sperm are assessed as genetically full.

EFFECT: use of the invention enables to improve the diagnostics of pathological disorders in sperm in the chromatin level.

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RU 2 568 845 C1

Authors

Trofimov Vladimir Aleksandrovich

Puzanov Sergej Jur'Evich

Trofimov Aleksandr Vladimirovich

Steblov Anton Pavlovich

Dates

2015-11-20Published

2014-05-30Filed