METHOD FOR PRODUCTION AND SELECTION OF MOLECULES INCLUDING, AT LEAST TWO DIFFERENT GROUPS, AND APPLICATION THEREOF Russian patent published in 2018 - IPC C07K1/107 C12P21/08 C12N9/52 C12R1/445 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Proposed is a method of a multi-specific binding molecule preparation comprising at least two binding moieties, such as an antibody, where the method comprises culturing a cell containing a nucleic acid encoding soluble A sortase from S. aureus with a C-terminal retention signal in the endoplasmic reticulum, nucleic acid encoding a first binding group comprising a sortase motif at its C-end, followed by a retention signal in the endoplasmic reticulum, and a nucleic acid encoding a second binding group containing at least one diglycine at its N-end. Said cell secrets the sortase A mediated conjugate of the first binding group and the second binding group.

EFFECT: present invention provides an enzyme-catalyzed preparation of a multi-specific binding molecule and can find further application, in particular, for the rapid creation of such binding molecules’ libraries.

7 cl, 4 dwg, 1 tbl, 3 ex