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2 575 621

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IPC

C12N1/21(2006-01-01)

C12N15/31(2006-01-01)

C12N15/70(2006-01-01)

(21) (22)

Application

2015100909/10, 2015-01-13

(24)

Start date

2015-01-13

(22)

Actual filing date

2015-01-13

(45)

Published

2016-02-20

(72)

Inventor

Dukhovlinov Il'Ja VladimirovichKadykov Aleksandr LeonidovichSimbirtsev Andrej Semenovich

(73)

Holder

Obshchestvo S Ogranichennoj Otvetstvennost'Ju

STRAIN-PRODUCER OF METHIONINE-FREE CRM197 BASED ON CELLS E. coli BL21 (DE3) Russian patent published in 2016 - IPC C12N1/21 C12N15/31 C12N15/70

Abstract RU 2575621 C1

FIELD: bioengineering.

SUBSTANCE: suggested strain is produced based on cells Escherichia coli BL21 (DE3) and contains plasmide DNA pETDuet-1, comprising the gene from which the protein CRM197 is produced, and additionally the gene is produced and used to synthesize methionine aminopeptidase E. coli, wherein the genes are codon optimised for expression in cells E. coli, and the synthesis of two proteins is performed simultaneously.

EFFECT: increased immunogenicity of vaccines.

1 dwg, 4 ex

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RU 2 575 621 C1

Authors

Dukhovlinov Il'Ja Vladimirovich

Kadykov Aleksandr Leonidovich

Simbirtsev Andrej Semenovich

Dates

2016-02-20—Published

2015-01-13—Filed